GSM1356707: KO H3K4me1 Input; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast
Attributes by original data submitter
Sample
source_name
KO MEF_input
cell type
primary mouse embryonic fibroblasts
strain
C57BL/6
h3k4me1 chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with formaldehyde and nuclear DNA was sonicated with 200-300bp, then the H3K4me1-DNA complexes were isolated with antibody. ChIP DNA libraries were made following Illumina ChIP-Seq library preparation kit and subjected to Solexa sequencing (Illumina) at the CCR-Sequencing Facility, National Cancer Institute. Sequencing was performed on Illumina Genome Analyzer IIx.