GSM3466331: biotin alone biotin ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Hela S3 cells
cell line
Hela S3 cells
genotype/variation
stably expressed biotin tag alone
antibody
n/a
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For biotin ChIP-seq experiments, cells stably expressed biotin-alone or biotin-CTCF or biotin-CTCF-s were expanded and crosslinked with 1% formaldehyde. Crosslinked cells were sonicated and diluted 10-fold with ChIP dilution buffer, and then incubated with M280 streptavidin dynabeads at 4°C for overnight. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols.