Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
lem-2

Cell type

Cell type Class
Embryo
Cell type
Early embryo
NA
NA

Attributes by original data submitter

Sample

source_name
early embryo
strain
set-25(n5021) III
antibody
Lem-2 antibody (Novus Biologicals 48540002)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were fixed with 2% Formalin for 30min at room temperature. Fixed chromatin was extracted using the FastPrep-24™ (MPI) homogenizer and sonicated using the Bioruptor (Diagenode) Libraries were prepared from chromatin IP and input samples using the TruSeq ChIP Library Preparation Kit (Illumina IP-202-1012)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

ce10

Number of total reads
17088064
Reads aligned (%)
119.3
Duplicates removed (%)
28.9
Number of peaks
439 (qval < 1E-05)

ce11

Number of total reads
17088064
Reads aligned (%)
119.3
Duplicates removed (%)
28.9
Number of peaks
434 (qval < 1E-05)

Base call quality data from DBCLS SRA