GSM3463656: FlagCDC20 Input rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK 293T cells
cell line
HEK 293T
overexpression
FlagCDC20
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was sheared by sonication and protein-DNA complexes were isolated with Flag M2 antibody (Sigma F1804). Libraries were generated using the Wafergen DNA prep kit (Illumina PrepX). Indexing and fragment enrichment were performed by PCR (Kapa HiFi PCR kit). DNA fragments were further size selected using SAGE Pippin Prep (<600 bp).