Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Monocytes
MeSH Description
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Attributes by original data submitter

Sample

source_name
Adult Human Peripheral Blood
cell type
Monocyte
treatment protocol
24 hours in media with oxLDL, then 5 days in media, then LPS for 4 hours
chip antibody
Rabbit polyclonal anti-H3K27ac (Diagenode, cat. # pAb-196-050)
donor
Healthy_Donor_1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Purified cells were fixed with 1% formaldehyde (Sigma) at a concentration of approximately 10 million cells/ml. Fixed cell preparations were sonicated using a Diagenode Bioruptor UCD-300 for 3x 10 minutes (30 seconds on; 30 seconds off). 33 µl of chromatin (1 ug) was used for immunoprecipitation. Libraries were prepared using the KAPA hyperprep kit.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
19256081
Reads aligned (%)
98.2
Duplicates removed (%)
37.6
Number of peaks
31104 (qval < 1E-05)

hg19

Number of total reads
19256081
Reads aligned (%)
97.8
Duplicates removed (%)
37.7
Number of peaks
31043 (qval < 1E-05)

Base call quality data from DBCLS SRA