Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me2

Cell type

Cell type Class
Neural
Cell type
Neural Stem Cells
MeSH Description
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Attributes by original data submitter

Sample

source_name
Neural stem cells (NSCs)
cell type
Neural stem cells (NSCs)
strain
C57BL/6J
developmental stage
E12.5
chip antibody
H3K9me2 (Abcam ab1220)
genotype
WT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For Chip-seq lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the NEBNext® Ultra DNA Library Prep for Illumina® kit (ref. E7370) according to the manufacturer's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
35959085
Reads aligned (%)
97.3
Duplicates removed (%)
16.3
Number of peaks
432 (qval < 1E-05)

mm9

Number of total reads
35959085
Reads aligned (%)
97.1
Duplicates removed (%)
16.3
Number of peaks
473 (qval < 1E-05)

Base call quality data from DBCLS SRA