Antigen

Antigen Class

TFs and others

Antigen

Phf2

Cell type

Cell type Class

Neural

Cell type

Neural Stem Cells

MeSH Description

Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Sample

source_name

Neural stem cells (NSCs)

cell type

Neural stem cells (NSCs)

strain

C57BL/6J

developmental stage

E12.5

chip antibody

PHF2 (Cell signaling D45A2)

genotype

WT

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For Chip-seq lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the NEBNext® Ultra DNA Library Prep for Illumina® kit (ref. E7370) according to the manufacturer's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

34392703

Reads aligned (%)

94.2

Duplicates removed (%)

19.8

Number of peaks

4587 (qval < 1E-05)

mm9

Number of total reads

34392703

Reads aligned (%)

94.0

Duplicates removed (%)

19.8

Number of peaks

4612 (qval < 1E-05)