GSM3455893: H3K4me3 ChIP-seq WT Day 7 - Replicate 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA
Attributes by original data submitter
Sample
source_name
Surface Ectoderm Cells
cell line
H9
genotype
wild-type
treatment strategy
Day 7 of differentiation with RA/BMP4 (surface ectoderm)
chip-seq antibody
H3K4me3 (Abcam ab8580)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde, chromatin lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with the indicated antibody. 60x10^6 and 10x10^6 cross-linked cells were used per assay for p63 or histone marks, respectively. DNA was quantified using Qubit (Invitrogen). Between 5 and 10 ng of pooled DNA were used for library preparation using the NEBNext kit (New England Biolabs) and Agencourt AMPure XP beads (Beckman), both according to the manufacturer's protocol.