Antigen

Antigen Class

TFs and others

Antigen

PIAS1

Cell type

Cell type Class

Prostate

Cell type

VCaP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

VCaP_PIAS1_EtOH

cell line

Vertebral Cancer of Prostate (VCaP)

cell line origin

bone metastasis of prostate cancer

treated with

Ethanol for 2hr

chip antibody

PIAS1

chip antibody vendor

Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were defined from sonicated nuclei and AR-, PIAS1-, FOXA1- and H3K4me2-DNA complexes were isolated with 1 mg of antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocol, as utilized at the Functional Genomics Unit, Helsinki, Finland and at the Gene Core in EMBL, Heidelberg, Germany.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

41367090

Reads aligned (%)

98.0

Duplicates removed (%)

4.3

Number of peaks

1539 (qval < 1E-05)

hg19

Number of total reads

41367090

Reads aligned (%)

97.1

Duplicates removed (%)

5.6

Number of peaks

1850 (qval < 1E-05)