RNA polymerase II (Santa Cruz, catalog# sc-899X, lot#B2713)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
U2932 cells were collected, cross-linked, sonicated and lyzed. Samples were immunoprecipitated with polyclonal antibodies specific for PRDM11, RNA polII (sc-899X, Santa Cruz) or rabbit IgG (Sigma). Eluted DNA was de-crosslinked and extracted using the QIAquick PCR Purification Kit (Qiagen) or MinElute PCR purification Kit (Qiagen) DNA libraries were build using NEBNext DNA Library Prep (NEB) according to manufacturer’s instructions