GSM3452791: XPW60-6 control CTCF ChIP-seq noguide; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293
perturbation/epigenome editor
control
grna
noguide
assay
ChIP-seq
chip epitope
CTCF
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Cells were crosslinked in 1% formaldehyde at 37C for 10min, quenched with glycine, lysed on ice in buffer containing 1% SDS, diluted to a final concentration of 0.3% SDS and sonicated for 5 minutes total time at ~10W power. Sonicated chromatin was incubated with the relevant antibody overnight at 4C with rotation after dilution to 0.1% SDS. ChIP-seq library prep: Performed as previously described using 5 ng (CTCF and H3K27ac), or 20 ng (H3K9me3) of ChIP DNA input. ChIP-seq sequencing: Paired-end 38 bp reads were generated on a NextSeq500 (Illumina).