GSM3452674: HHV6-GFP TRF1 ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
TRF1 (GeneTex, cat#32935)
Sequenced DNA Library
ChIP-seq: Cells were fixed with 1% formaldehyde for 10 minutes at room temperature and then quenched with 0.125M glycine for 5 minutes, prior to several washes with ice-cold PBS. Cells were lysed and IPs were performed with the indicated antibody at a ratio of 4ug antibody to 20ug chromatin (for histone modifications) and 10ug antibody to 150ug chromatin (for TRF1/2). ChIP-seq libraries were prepared with the NEB Ultra II Library Prep Kit following the manufacturer´s protocol.