GSM3452674: HHV6-GFP TRF1 ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TERF1
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HHV6-GFP cells
infection
HHV6 infected
cell type
HEK293 cells
chip antibody
TRF1 (GeneTex, cat#32935)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Cells were fixed with 1% formaldehyde for 10 minutes at room temperature and then quenched with 0.125M glycine for 5 minutes, prior to several washes with ice-cold PBS. Cells were lysed and IPs were performed with the indicated antibody at a ratio of 4ug antibody to 20ug chromatin (for histone modifications) and 10ug antibody to 150ug chromatin (for TRF1/2). ChIP-seq libraries were prepared with the NEB Ultra II Library Prep Kit following the manufacturer´s protocol.