GSM1350483: Flag-Rorc EL4 no exogeneous Rorc (control); Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Blood
Cell type
EL4
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma
Attributes by original data submitter
Sample
source_name
EL4 cells
strain
C57BL/6
cell type
EL-4 cells
antibody
Anti-Flag, Sigma, F3165
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
~50-100 x 106 cells for each ChIP were crosslinked with formaldehyde, lysed, and fragmented with a Misonix Sonicator 3000. Following immunoprecipitation with antibody or IgG control, the protein-DNA crosslinks were reversed and DNA was purified and used for the preparation of Illumina sequencing libraries Purified ChIP DNA was used to prepare ChIP-seq libraries using the Illumina TruSeq DNA Sample Preparation v2 kit