Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
Th17 Cells
MeSH Description
A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Attributes by original data submitter

Sample

source_name
Th17 cells
strain
C57BL/6
cell type
Th17 cells
genotype/variation
wildtype
antibody
rat IgG2a, eBioscience, 14-6988,

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
~50-100 x 106 cells for each ChIP were crosslinked with formaldehyde, lysed, and fragmented with a Misonix Sonicator 3000. Following immunoprecipitation with antibody or IgG control, the protein-DNA crosslinks were reversed and DNA was purified and used for the preparation of Illumina sequencing libraries Purified ChIP DNA was used to prepare ChIP-seq libraries using the Illumina TruSeq DNA Sample Preparation v2 kit

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
28234376
Reads aligned (%)
72.3
Duplicates removed (%)
15.0
Number of peaks
759 (qval < 1E-05)

mm9

Number of total reads
28234376
Reads aligned (%)
72.1
Duplicates removed (%)
15.1
Number of peaks
866 (qval < 1E-05)

Base call quality data from DBCLS SRA