GSM3446315: MED16 CHIP-seq in USP22 knockdown (shUSP22), untreated cells, replicate 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MED16
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116 colorectal cancer cells
cell line
HCT116
factor
MED16
usp22 status
KO
shRNA
shUSP22
treatment
u
replicate
1
antibody
Abcam cat# ab130996
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 10 minutes at room temperature, followed by quenching with 0.18 M Glycine for 5 minutes. Cells were harvested and chromatin was extracted 10 ng of DNA from Inputs and IPs were used to prepare libraries using Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences, MI) following manufacturer's protocol with an additional wash at the end using SPRIselect beads (Beckman Coulter, IN) to remove any larger libraries that could interfere with clustering on the instrument