Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryo

Cell type

Mesoderm

NA

NA

Sample

source_name

mesoderm stage 14-17

cell type

mesoderm

chip-antibody

none

genotype

twi-INTACT; UAS-Nslmb,GFP-Ubx

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

tissue specific nuclei were extracted vis the INTACT method (Steiner et al., 2012) Samples were amplified using the ThruPLEX DNA-Seq Kit (Rubicon). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

28078010

Reads aligned (%)

96.9

Duplicates removed (%)

17.7

Number of peaks

2887 (qval < 1E-05)

dm3

Number of total reads

28078010

Reads aligned (%)

97.6

Duplicates removed (%)

14.3

Number of peaks

1790 (qval < 1E-05)