Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
Mesoderm
NA
NA

Attributes by original data submitter

Sample

source_name
mesoderm stage 14-17
cell type
mesoderm
chip-antibody
H3K27me3 (ab6002, Abcam)
genotype
twi-INTACT; UAS-Nslmb,GFP-Ubx

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
tissue specific nuclei were extracted vis the INTACT method (Steiner et al., 2012) Samples were amplified using the ThruPLEX DNA-Seq Kit (Rubicon). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
21326443
Reads aligned (%)
81.0
Duplicates removed (%)
25.9
Number of peaks
1616 (qval < 1E-05)

dm6

Number of total reads
21326443
Reads aligned (%)
80.4
Duplicates removed (%)
28.2
Number of peaks
2304 (qval < 1E-05)

Base call quality data from DBCLS SRA