Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ubx

Cell type

Cell type Class
Embryo
Cell type
Mesoderm
NA
NA

Attributes by original data submitter

Sample

source_name
mesoderm stage 10-13
cell type
mesoderm
chip-antibody
gp-Ubx (home made)
genotype
twi-INTACT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
tissue specific nuclei were extracted vis the INTACT method (Steiner et al., 2012) Samples were amplified using the ThruPLEX DNA-Seq Kit (Rubicon). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
31010306
Reads aligned (%)
63.5
Duplicates removed (%)
35.3
Number of peaks
0 (qval < 1E-05)

dm6

Number of total reads
31010306
Reads aligned (%)
57.2
Duplicates removed (%)
41.3
Number of peaks
4309 (qval < 1E-05)

Base call quality data from DBCLS SRA