SRX4906178
GSM3438406: ChIPseq-Th2-Setdb1-KO-H3K27ac-experiment2; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Spleen CD4+ T cells
cell type
Th2 T lymphocytes
number of culture days
6
strain
C57BL/6 Setdb1-/-
antibody
H3K27ac (ab4729 from Abcam; lot GR228556-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA purification was performed by column (MinElute PCR purification kit, Qiagen). Libraries were prepared for sequencing using standard TruSeq Illumina protocols (ChIP-seq) and Nextera DNA Library Prep Kit Illumina (ATAC-seq).

Sequencing Platform

instrument_model
Illumina HiSeq 3000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
43847936
Reads aligned (%)
91.4
Duplicates removed (%)
21.0
Number of peaks
9046 (qval < 1E-05)

mm9

Number of total reads
43847936
Reads aligned (%)
91.3
Duplicates removed (%)
20.9
Number of peaks
9003 (qval < 1E-05)

Base call quality data from DBCLS SRA