Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

mESC derived neural cells

NA

NA

Sample

source_name

EB-Motor neuron, WT, IgG control ChIP

strain/background

C57BL/6

genotype/variation

wild type

cell type

EB-Motor neuron

chip antibody

IgG

cell line

HBG3 clone

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq: EB-MN were fixed with 0.5% PFA and ChIP was performed. See details in associated manuscript (Herrera et al.) Libraries were constructed following Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

176672613

Reads aligned (%)

78.1

Duplicates removed (%)

61.7

Number of peaks

1075 (qval < 1E-05)

mm9

Number of total reads

176672613

Reads aligned (%)

77.9

Duplicates removed (%)

61.7

Number of peaks

1289 (qval < 1E-05)