Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Epidermis
Cell type
BJ
Primary Tissue
Skin
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
BJ_DNA damage_p53
cell line
BJ
cell type
Human diploid foreskin fibroblasts (BJ) immortalized with hTERT
exposed to
10 Gy of ionizing radiation (IR)
chip antibody
p53 (DO-1)
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The JMJD3 antibody was generated in rabbits, using affinity-purified GST-JMJD3 (amino acids 798 1095) as described in (PMID 19451217). Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
85221360
Reads aligned (%)
46.6
Duplicates removed (%)
48.6
Number of peaks
3076 (qval < 1E-05)

hg38

Number of total reads
85221360
Reads aligned (%)
48.1
Duplicates removed (%)
47.3
Number of peaks
3207 (qval < 1E-05)

Base call quality data from DBCLS SRA