GSM3436208: insR DMSO H3K27AC; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
SET-2
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
insR SET-2 cells, DMSO, 48 hours
cell line
SET-2
genotype/variation
drug-resistant SET-2 LSD1 (Leu659_Asn660insArg)
treatment
DMSO
cell type
acute myeloid leukemia
chip antibody
AB_2561016 (H3K27ac-mouse antibody, Active Motif 39133, LOT# 31814008)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde, washed, snap frozen, and stored at -80ºC. Sonication of crosslinked cells was calibrated such that DNA was sheared to between 300bp and 2000bp. ChIP DNA was used to generate sequencing libraries by end repair (End-It DNA repair kit, Epicentre), 3' A base overhang addition via Klenow fragment (NEB), and ligation of barcoded sequencing adapters.