Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GFI1B

Cell type

Cell type Class
Blood
Cell type
SET-2
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
WT SET-2 cells, DMSO, 48 hours
cell line
SET-2
genotype/variation
wild type
treatment
DMSO
cell type
acute myeloid leukemia
chip antibody
AB_449000 (GFI1B antibody, Abcam ab26132, LOT# GR318193-8)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde, washed, snap frozen, and stored at -80ºC. Sonication of crosslinked cells was calibrated such that DNA was sheared to between 300bp and 2000bp. ChIP DNA was used to generate sequencing libraries by end repair (End-It DNA repair kit, Epicentre), 3' A base overhang addition via Klenow fragment (NEB), and ligation of barcoded sequencing adapters.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
0
Reads aligned (%)
0.0
Duplicates removed (%)
8.1
Number of peaks
2125 (qval < 1E-05)

hg38

Number of total reads
0
Reads aligned (%)
0.0
Duplicates removed (%)
7.9
Number of peaks
2298 (qval < 1E-05)

Base call quality data from DBCLS SRA