Harvested lysates were fixed in 1% MeOH formaldehyde and sonicated. Following sonication, samples were incubated with RUNX1 ab for ChIP reaction. Ab-DNA complex isolated using Protein A/G beads and magnets. DNA was purified from solution and subject to library construction ChIP libraries were generated using Accel-NGS® 2S Plus DNA Library kit (Swift Biosciences, Ann Arbor, MI) following manufacturers protocol. Input and RUNX1 ChIP samples were normalized to 1ng prior to library generation. Libraries were amplified in an optional PCR step for 12 total cycles. Finalized libraries were double size selected using AMPure XP beads (0.8X and 0.2X volume ratios to sample), resulting in the majority fragments sized between 250-400bp.