Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HOXB8

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
cell line
K562
cell type
myelogenous leukemia cell line
passages
5-10
chip antibody
HOXB8 antibody (Abcam, cat# ab125727)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and transcription factor-DNA complexes were isolated with a HOXB8 antibody. Libraries of purified ChIP DNA for Illumina sequencing were prepared using the Illumina TruSeq DNA Sample Preparation v2 kit according to manufacturer's instructions.

Sequencing Platform

instrument_model
HiSeq X Ten

hg19

Number of total reads
22868326
Reads aligned (%)
77.9
Duplicates removed (%)
13.9
Number of peaks
4062 (qval < 1E-05)

hg38

Number of total reads
22868326
Reads aligned (%)
79.1
Duplicates removed (%)
13.2
Number of peaks
4055 (qval < 1E-05)

Base call quality data from DBCLS SRA