Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

hemin induced K562 cell line

cell line

K562

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The cells were cross-linked with 1% formaldehyde for 10 mins at room temperature and quenched with 125 mM glycine. The cells were washed in PBS and then lysed in buffer containing 1% to 0.1% SDS. The genomic DNA was sonicated to 200 to 1000 bps and the biotin-protein-DNA complexes ware precipitated with Dynabeads™ M-280 Streptavidin (Thermo Cat# 11205D) at 4°C for 2 hours, followed by washing and de-crosslinking and DNA purification for subsequent analysis Fragmented IP'ed DNA samples were processed using the Beckman Coulter SPRIworks system to generate libraries. Resulting libraries were sequenced on the HiSeq2000.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

61046108

Reads aligned (%)

98.7

Duplicates removed (%)

77.1

Number of peaks

1073 (qval < 1E-05)

hg19

Number of total reads

61046108

Reads aligned (%)

98.2

Duplicates removed (%)

78.5

Number of peaks

1047 (qval < 1E-05)