Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
rhi

Cell type

Cell type Class
Adult
Cell type
Ovary
NA
NA

Attributes by original data submitter

Sample

source_name
Rhino ChIP from piwi germline knock-down ovaries
developmental stage
4-6 days old adult
Sex
female
tissue
ovary
germline knock-down
piwi
chip antibody
custom-made rabbit polyclonal antibody

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed according to Lee et al, Nat Protoc, 2006 ChIP-seq libraries were generated using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) and the Kapa HiFi Library Amp Real-Time Kit (Peqlab) for amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
19281031
Reads aligned (%)
21.6
Duplicates removed (%)
52.4
Number of peaks
2892 (qval < 1E-05)

dm6

Number of total reads
19281031
Reads aligned (%)
20.0
Duplicates removed (%)
55.6
Number of peaks
3170 (qval < 1E-05)

Base call quality data from DBCLS SRA