Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Blood
Cell type
T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Foxp3GFPKO (male)
cell type
Foxp3GFPKO (male)
antibody
H3K27me3 antibody (Upstate 07-449)
cell source
Primary Mouse T cells isolated ex-vivo and sorted

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were harvested from mice that were approximately 8 weeks old. Lymph nodes and spleen were extracted and sorted based on GFP (Foxp3) Cells were fixed with formaldehyde, nuclei were extracted and sonicated. Antibodies were incubated with sonicated chromatin. Standard illumina kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
32363260
Reads aligned (%)
83.5
Duplicates removed (%)
26.0
Number of peaks
433 (qval < 1E-05)

mm9

Number of total reads
32363260
Reads aligned (%)
83.3
Duplicates removed (%)
26.0
Number of peaks
454 (qval < 1E-05)

Base call quality data from DBCLS SRA