Foxp3 antibody is a custom polyclonal antibody that has been affinity purified
cell source
Primary Mouse T cells isolated ex-vivo and sorted
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were harvested from mice that were approximately 8 weeks old. Lymph nodes and spleen were extracted and sorted based on GFP (Foxp3) Cells were fixed with formaldehyde, nuclei were extracted and sonicated. Antibodies were incubated with sonicated chromatin. Standard illumina kit