GSM3425381: NALM6 H3K4ME3 DOX; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Blood
Cell type
NALM-6
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
NALM6
cell type
B-ALL cell line
cell line
NALM6
antibody
H3K4me3 (ab8580, Abcam)
treatment
DOX
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Exactly ten million cells were pelleted and fixed with formaldehyde. Nuclei were harvested upon cell lysis, at which time drosophila S2 cell nuclei were spiked into cells in lysis buffer at one S2 cell nucleus per five experimental cells. Probe sonication (micro tip, 30 amplitude, 30” on, 8 cycles) was optimized for Nalm6 cells. Special attention was paid to equal and consistent volume transfers. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.