Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Cell line
Cell type
OSC
Tissue Source
ovary
Developmental Stage
adult stage

Attributes by original data submitter

Sample

source_name
OSCs
cell type
OSC
genotype
Piwi knockdown
chip antibody
anti-H3K9me3 antibody produced in rabbit (Active Motif Cat# 39161)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was carried out according to Lee et al. (2006) with NEBNext Ultra DNA Library Prep Kit Illumina (NEB)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm6

Number of total reads
39540484
Reads aligned (%)
84.3
Duplicates removed (%)
35.6
Number of peaks
0 (qval < 1E-05)

dm3

Number of total reads
39540484
Reads aligned (%)
84.9
Duplicates removed (%)
27.2
Number of peaks
12331 (qval < 1E-05)

Base call quality data from DBCLS SRA