Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
primary CD4 T-cells
chip antibody
H3K27ac (Abcam, cat# ab4729, lot# GR3198866-1)
cell type
primary CD4 T-cells
t-cell status
resting

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the iDeal ChIP-qPCR Kit (Diagenode, Cat# C01010180). Each ChIP reaction was performed on 1 × 10 6 cells. Sonication was performed at 30s in eight cycles (Bioruptor Pico, Diagenode, Cat# B01060010). ChIP antibody was targeting H3K27ac (Abcam, Cat# ab4729). Input samples were taken from same sample before ChIP. NEB Ultra II DNA library kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
26235145
Reads aligned (%)
83.5
Duplicates removed (%)
19.7
Number of peaks
6510 (qval < 1E-05)

hg19

Number of total reads
26235145
Reads aligned (%)
82.8
Duplicates removed (%)
20.3
Number of peaks
6688 (qval < 1E-05)

Base call quality data from DBCLS SRA