GSM3425286: H3K27ac ChIP of resting T-cells; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
primary CD4 T-cells
chip antibody
H3K27ac (Abcam, cat# ab4729, lot# GR3198866-1)
cell type
primary CD4 T-cells
t-cell status
resting
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the iDeal ChIP-qPCR Kit (Diagenode, Cat# C01010180). Each ChIP reaction was performed on 1 × 10 6 cells. Sonication was performed at 30s in eight cycles (Bioruptor Pico, Diagenode, Cat# B01060010). ChIP antibody was targeting H3K27ac (Abcam, Cat# ab4729). Input samples were taken from same sample before ChIP. NEB Ultra II DNA library kit