Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP (ATCC number:CRL-1740)
antibody
Androgen Receptor (06-680, Millipore, Lot number 2746312)
protein
Androgen Receptor
cell type
Metastatic, Prostate cancer cell from left supraclavicular lymph node from Lymph node
cell line
LNCaP

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin Immunoprecpitations were performed using HighCell ChIP kit following manufacturer's protocol (Diagenode). The ChIP DNA was isolated and purified (IPure Kit, Diagenode) from samples. Next- generation sequencing library preparation using KAPA Hyper Prep kit (KAPA Biosystems) using TruSeq adapters (Integrated DNA Technologies). A quality check of the purified libraries was carried out using the Bioanalyzer 2100 (Agilent).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
83915780
Reads aligned (%)
77.9
Duplicates removed (%)
8.0
Number of peaks
19167 (qval < 1E-05)

hg38

Number of total reads
83915780
Reads aligned (%)
80.1
Duplicates removed (%)
6.5
Number of peaks
19918 (qval < 1E-05)

Base call quality data from DBCLS SRA