CTCF antibody (3418S Cell Signaling Technology, Lot number 3
protein
CTCF
cell type
Metatstatic, Prostate cancer cells from Brain
cell line
DU145
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin Immunoprecpitations were performed using HighCell ChIP kit following manufacturer's protocol (Diagenode). The ChIP DNA was isolated and purified (IPure Kit, Diagenode) from samples. Next- generation sequencing library preparation using KAPA Hyper Prep kit (KAPA Biosystems) using TruSeq adapters (Integrated DNA Technologies). A quality check of the purified libraries was carried out using the Bioanalyzer 2100 (Agilent).