Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Prostate
Cell type
DU 145
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
DU145 (ATCC number:HTB-81)
antibody
CTCF antibody (3418S Cell Signaling Technology, Lot number 3
protein
CTCF
cell type
Metatstatic, Prostate cancer cells from Brain
cell line
DU145

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin Immunoprecpitations were performed using HighCell ChIP kit following manufacturer's protocol (Diagenode). The ChIP DNA was isolated and purified (IPure Kit, Diagenode) from samples. Next- generation sequencing library preparation using KAPA Hyper Prep kit (KAPA Biosystems) using TruSeq adapters (Integrated DNA Technologies). A quality check of the purified libraries was carried out using the Bioanalyzer 2100 (Agilent).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
52829386
Reads aligned (%)
86.6
Duplicates removed (%)
10.0
Number of peaks
30888 (qval < 1E-05)

hg38

Number of total reads
52829386
Reads aligned (%)
89.0
Duplicates removed (%)
8.5
Number of peaks
31378 (qval < 1E-05)

Base call quality data from DBCLS SRA