CTCF antibody (3418S Cell Signaling Technology, Lot number 3
Metatstatic, Prostate cancer cells from Brain
Sequenced DNA Library
Chromatin Immunoprecpitations were performed using HighCell ChIP kit following manufacturer's protocol (Diagenode). The ChIP DNA was isolated and purified (IPure Kit, Diagenode) from samples. Next- generation sequencing library preparation using KAPA Hyper Prep kit (KAPA Biosystems) using TruSeq adapters (Integrated DNA Technologies). A quality check of the purified libraries was carried out using the Bioanalyzer 2100 (Agilent).