GSM1340092: MEL18 OHT HS ChipSeq technical replicate 1 and 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
PCGF2
Cell type
Cell type Class
Others
Cell type
Fibroblasts
NA
NA
Attributes by original data submitter
Sample
source_name
Cultured fibroblasts
genotype
normal foreskin
chip antibody
MEL18 (Santa Cruz sc-10744)
treatment
300 nM 4-hydroxy tamoxifen (OHT) for 4 h
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA samples were end repaired, poly-A tailed and Illumina single end adapters were ligated following the standard Illumina protocol with minor adjustments. Agencourt AMPure XP beads at 0.8x ratio were used to size select out adapter dimers after adapter ligation. The Illumina kit Phusion enzyme was replaced by Kapa HiFi HotStart ready mix. Post PCR, AMPure XP beads were used at a 1:1 ratio to maintain size integrity and to allow use of the Invitrogen SizeSelect E-gel system. Samples were finally purified with QAIquick gel extraction kit and quality controlled on the DNA 1000 BioAnalyser 2100 chip before clustering and subsequent 50-51bp single end sequencing on the Illumina HiSeq 2500.