Sheared Chromatin were cleared and protein-DNA complexes were isolated with antibody-magnetic bead complex. ChIP-seq library for each sample were prepared according to manufacturer (NEXTFLEX® ChIP-Seq Kit 5143-01) protocols. Briefly,purified immunoprecipitated DNA were end-repaired and size selected for 200–400-bp fragments with Agencourt AMPure XP beads (A63880) followed by Adenylation, barcoded adaptor-ligation and PCR amplification (15 cycles). Size distribution and concentration of each library was checked using Agilent Bioanalyzer. Equivalent amounts of barcoded libraries were pooled and sequenced using HiSeq 2500 (Illumina) instruments.