Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EPAS1

Cell type

Cell type Class
Kidney
Cell type
HKC8
NA
NA

Attributes by original data submitter

Sample

source_name
kidney proximal tubule cell line
cell line
HKC8
tissue
kidney proximal tubule cell line
degree of hypoxia
0.5%
duration of hypoxia
16 hours
chip antibody
HIF2a (PM9)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) experiments were performed using antibodies directed against HIF-1a (rabbit polyclonal, PM14), HIF-2a (rabbit polyclonal, PM9), or HIF-1b (rabbit polyclonal, Novus Biologicals, NB100-110). Libraries were prepared from immunoprecipitated chromatin using the Illumina ChIP-Seq kit All ChIP-seq experiments were performed in duplicate in accordance with ENCODE consortium guidelines

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
8161768
Reads aligned (%)
95.5
Duplicates removed (%)
2.6
Number of peaks
279 (qval < 1E-05)

hg38

Number of total reads
8161768
Reads aligned (%)
97.1
Duplicates removed (%)
1.7
Number of peaks
212 (qval < 1E-05)

Base call quality data from DBCLS SRA