GSM3417780: HKC8 0.5 6hr HIF2a rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EPAS1
Cell type
Cell type Class
Kidney
Cell type
HKC8
NA
NA
Attributes by original data submitter
Sample
source_name
kidney proximal tubule cell line
cell line
HKC8
tissue
kidney proximal tubule cell line
degree of hypoxia
0.5%
duration of hypoxia
6 hours
chip antibody
HIF2a (PM9)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) experiments were performed using antibodies directed against HIF-1a (rabbit polyclonal, PM14), HIF-2a (rabbit polyclonal, PM9), or HIF-1b (rabbit polyclonal, Novus Biologicals, NB100-110). Libraries were prepared from immunoprecipitated chromatin using the Illumina ChIP-Seq kit All ChIP-seq experiments were performed in duplicate in accordance with ENCODE consortium guidelines