Antigen

Antigen Class

TFs and others

Antigen

Rbp1

Cell type

Cell type Class

Cell line

Cell type

S2R+

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

ChIP Seq of total Pol II in Ctrl Knockdown condition (earlier passage)

cell line

S2R+ Cells

cell type

Embryonic

genotype/variation

control knockdown

chip antibody

RBP1 (Diagenode Cat No-15200004) / Lot No 001-11

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 _l of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA library preperation kit, following manufacturer's protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm3

Number of total reads

11648449

Reads aligned (%)

97.0

Duplicates removed (%)

20.8

Number of peaks

8881 (qval < 1E-05)

dm6

Number of total reads

11648449

Reads aligned (%)

96.9

Duplicates removed (%)

22.8

Number of peaks

8523 (qval < 1E-05)