Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Rbp1

Cell type

Cell type Class
Cell line
Cell type
S2R+
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
ChIP Seq of total Pol II in Ctrl Knockdown condition (earlier passage)
cell line
S2R+ Cells
cell type
Embryonic
genotype/variation
control knockdown
chip antibody
RBP1 (Diagenode Cat No-15200004) / Lot No 001-11

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 _l of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA library preperation kit, following manufacturer's protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
11180850
Reads aligned (%)
96.7
Duplicates removed (%)
20.5
Number of peaks
8840 (qval < 1E-05)

dm6

Number of total reads
11180850
Reads aligned (%)
96.6
Duplicates removed (%)
22.5
Number of peaks
8461 (qval < 1E-05)

Base call quality data from DBCLS SRA