Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cell line

Cell type

S2R+

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

Input sample for Ctrl knockdown (earlier passage)

cell line

S2R+ Cells

cell type

Embryonic

genotype/variation

control knockdown

chip antibody

Input Sample

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 _l of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA library preperation kit, following manufacturer's protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

10967021

Reads aligned (%)

96.9

Duplicates removed (%)

12.9

Number of peaks

2630 (qval < 1E-05)

dm3

Number of total reads

10967021

Reads aligned (%)

97.4

Duplicates removed (%)

9.8

Number of peaks

2920 (qval < 1E-05)