Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
mago

Cell type

Cell type Class
Cell line
Cell type
S2R+
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
ChIP Seq of Mago HA with Mago KD condition
cell line
S2R+ Cells
cell type
Embryonic
chip antibody
HA-probe Antibody (F-7) (Santa Cruz, catalog# sc-7392, lot# I0916)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 _l of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA library preperation kit, following manufacturer's protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
17754733
Reads aligned (%)
97.8
Duplicates removed (%)
8.4
Number of peaks
4591 (qval < 1E-05)

dm6

Number of total reads
17754733
Reads aligned (%)
97.5
Duplicates removed (%)
12.2
Number of peaks
3600 (qval < 1E-05)

Base call quality data from DBCLS SRA