Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Neuroblastoma
MeSH Description
A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51)

Attributes by original data submitter

Sample

source_name
Neuroblastoma cells
strain/cell line
BE2C
genotype
N-myc kd
chip antibody
cell signaling 2729

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from nuclei by MNase and histone-DNA complexes were isolated with antibody Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
23446543
Reads aligned (%)
96.3
Duplicates removed (%)
1.3
Number of peaks
914 (qval < 1E-05)

hg19

Number of total reads
23446543
Reads aligned (%)
95.2
Duplicates removed (%)
1.9
Number of peaks
480 (qval < 1E-05)

Base call quality data from DBCLS SRA