GSM3416382: P4 Adipose PPARg ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
PPARG
Cell type
Cell type Class
Adipocyte
Cell type
Adipose Tissue
MeSH Description
Encapsulated adipose tissue.
Attributes by original data submitter
Sample
source_name
Adipose tissue
tissue
Adipose
chip antibody
PPARg(Santa Cruz Biotechnology, Cat# sc-7196)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted by using Qiagen RNeasey Mini kit. Ribosomal RNA was depleted by Ribo-Zero gold kit. DNA was isolated using phenol/chloroform extraction and NaCl/EtOH precipitated with 20 μg glycogen carrier. RNA libraries were prepared for sequencing using TruSeq RNA Sample Prep Kit. ChIP-seq library was prepared for sequencing according to the amplification protocol from Illumina.