Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PPARG

Cell type

Cell type Class
Adipocyte
Cell type
Adipose Tissue
MeSH Description
Encapsulated adipose tissue.

Attributes by original data submitter

Sample

source_name
Adipose tissue
tissue
Adipose
chip antibody
PPARg(Santa Cruz Biotechnology, Cat# sc-7196)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted by using Qiagen RNeasey Mini kit. Ribosomal RNA was depleted by Ribo-Zero gold kit. DNA was isolated using phenol/chloroform extraction and NaCl/EtOH precipitated with 20 μg glycogen carrier. RNA libraries were prepared for sequencing using TruSeq RNA Sample Prep Kit. ChIP-seq library was prepared for sequencing according to the amplification protocol from Illumina.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
44248847
Reads aligned (%)
53.6
Duplicates removed (%)
42.7
Number of peaks
14114 (qval < 1E-05)

hg38

Number of total reads
44248847
Reads aligned (%)
55.2
Duplicates removed (%)
41.4
Number of peaks
14625 (qval < 1E-05)

Base call quality data from DBCLS SRA