SRX4796164
GSM3416308: e12-2-H3K14Ac; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K14ac

Cell type

Cell type Class
Blood
Cell type
Hematopoietic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
MLL-AF9 I-BET resistant cells
strain/background
C57BL/6
cell type
haematopoietic progenitors
condition
I-BET Resistant
treatment
e12_2_HBO1_sgRNA
chip antibody
Acetyl-Histone H3(K14) (D4B9, Cell Signalling Technologies, ref 10/2017)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate was isolated from formaldehyde cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody. ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics).

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
10627574
Reads aligned (%)
94.8
Duplicates removed (%)
9.3
Number of peaks
27417 (qval < 1E-05)

mm9

Number of total reads
10627574
Reads aligned (%)
94.7
Duplicates removed (%)
9.3
Number of peaks
27412 (qval < 1E-05)

Base call quality data from DBCLS SRA