Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

Hematopoietic progenitors

NA

NA

Sample

source_name

MLL-AF9 I-BET resistant cells

strain/background

C57BL/6

cell type

haematopoietic progenitors

condition

I-BET Resistant

treatment

e12_2_HBO1_sgRNA

chip antibody

anti-RNA polymerase II (CTD4H8, Millipore, lot 2810725)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysate was isolated from formaldehyde cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody. ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics).

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

8004737

Reads aligned (%)

93.3

Duplicates removed (%)

15.3

Number of peaks

336 (qval < 1E-05)

mm9

Number of total reads

8004737

Reads aligned (%)

93.0

Duplicates removed (%)

15.4

Number of peaks

324 (qval < 1E-05)