Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
Hematopoietic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
MLL-AF9 I-BET resistant cells
strain/background
C57BL/6
cell type
haematopoietic progenitors
condition
I-BET Resistant
treatment
e11_1_HBO1_sgRNA
chip antibody
anti-RNA polymerase II (CTD4H8, Millipore, lot 2810725)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate was isolated from formaldehyde cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody. ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics).

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
9019252
Reads aligned (%)
92.6
Duplicates removed (%)
11.4
Number of peaks
1891 (qval < 1E-05)

mm9

Number of total reads
9019252
Reads aligned (%)
92.4
Duplicates removed (%)
11.6
Number of peaks
1886 (qval < 1E-05)

Base call quality data from DBCLS SRA