Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Adipocyte

Cell type

Brown preadipocytes

NA

NA

Sample

source_name

brown preadipocytes, input

strain/background

C57BL/6J

cell type

immortalized brown preadipocytes

passages

10

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Antibody against H3K27me3 (Millipore, Cat# 07-449, Lot# 2194165). 7.5 milllion cells were used for each ChIP. Illumina's ChIP-seq Sample Prep kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

18689657

Reads aligned (%)

78.2

Duplicates removed (%)

13.7

Number of peaks

277 (qval < 1E-05)

mm9

Number of total reads

18689657

Reads aligned (%)

77.9

Duplicates removed (%)

13.6

Number of peaks

269 (qval < 1E-05)