Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
prostate cancer cells
cell line
LNCaP
cell type
prostate cancer cells
chip antibody
AR (Santa Cruz, sc-816)
genotype/variation
shGREB1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and AR-DNA complexes were isolated with antibody. Libraries were made using the KAPA Biosystems Hyper Library Prep Kit (Kapa Biosystems, Woburn, MA, KK8504), using 10 ng of DNA as input and 10 PCR cycles for library amplification.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
32190917
Reads aligned (%)
63.9
Duplicates removed (%)
66.5
Number of peaks
881 (qval < 1E-05)

hg38

Number of total reads
32190917
Reads aligned (%)
65.8
Duplicates removed (%)
65.8
Number of peaks
993 (qval < 1E-05)

Base call quality data from DBCLS SRA