Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Blood

Cell type

PreB-ALL

NA

NA

Sample

source_name

Childhood acute lymphoblastic leukemia

subject status

Childhood acute lymphoblastic leukemia

subject id

883, 898; Pool of two patients

cell type

Pre-B t(12;21) [ETV6-RUNX1] acute lymphoblastic leukemia

chip antibody

Abcam; ab8895

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Mononuclear cells were isolated from bone marrow (BM) aspirates or peripheral blood cells at diagnosis and CD10+CD19+ cells were isolated from human cord blood WGBS libraries were carried out using the TruSeq DNA Sample Prep Kit v2 (Illumina) with an added bisulfite conversion step. ChIP-seq libraries were carried out using the Paired-End DNA Sample Prep Kit V1 (Illumina; PE-102-1001)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

63040185

Reads aligned (%)

81.3

Duplicates removed (%)

6.7

Number of peaks

2729 (qval < 1E-05)

hg19

Number of total reads

63040185

Reads aligned (%)

80.6

Duplicates removed (%)

7.0

Number of peaks

1818 (qval < 1E-05)